BioSqueeze
Butte, Montana, United States
BioSqueeze
!
Eliminating Sustained Casing Pressure. The Most Effective Solution to Eliminate Sustained Casing Pressure.
About BioSqueeze
For more than 8 years, BioSqueeze Inc.’s revolutionary biomineralization technology has been in the works. Its success stems from a unique, collaborative partnership between government, university, and industry experts. In 2014, the Montana State University Center for Biofilm Engineering (CBE) was given $8 million from the Department of Energy to research how microbes could be used to seal problematic defects in wellbore casing cement. The research team, including BioSqueeze Inc. founders Robert Hyatt and Randy Hiebert, found that naturally occurring soil bacteria could form crystalline calcium carbonate that would readily attach to rock, cement, and steel to form a permanent bond.
Innovative Technology
Through this research, the technology behind BioSqueeze® was born. Our founders brought the technology from the lab to the field, developing highly precise and sensitive equipment to make BioSqueeze® even more successful. In 2019, BioSqueeze Inc. sealed its first oil and gas well, successfully eliminating sustained casing pressure and pioneering the latest innovation in the oil and gas industry.
Proven Solution
Today, BioSqueeze® is quickly becoming known as the most effective solution for addressing sustained casing pressure, having successfully sealed leaks in nine U.S. states and two Canadian provinces. Leaks that were once impossible to seal now have a powerful solution, saving oil and gas operators millions of dollars and months of rig time that would otherwise be thrown at futile attempts to seal. BioSqueeze® has many potential applications for the oil and gas industry, and we’ll continue to bring new solutions to the table as leaders in biomineralization.
Description of Services
What is BioSqueeze®?
BioSqueeze® uses a low-viscosity, environmentally friendly biomineralizing solution to seal even the smallest leakage pathways, which no other technology can reach. Once in place, it forms a gas-tight crystalline calcium carbonate seal – similar to limestone – that readily bonds to rock, cement, and steel, permanently sequestering escaping gases at their source. BioSqueeze® harnesses the process of biomineralization in a new, innovative way, using naturally-occurring soil bacteria to create new minerals that solve problems for the oil and gas industry.
Biomineralization: A natural, effective way to stop methane leaks.
BioSqueeze Inc. is the world leader in biomineralization – the process by which living organisms produce minerals. Biomineralization is a safe, environmentally friendly technology that’s at the core of the BioSqueeze® solution and all of our applications.
How Does Biomineralization Work?
1. Biomineralization combines non-hazardous, naturally occurring soil bacteria and common, widely available, and safe chemicals used as food additives, fertilizers, and agricultural feed products. Together, they create a fluid with the ability to transform into new minerals.
2. This environmentally friendly mineralizing solution is pumped deep into leakage pathways of a well. With a viscosity of 1.05 cP – similar to water – our solution can easily penetrate tiny micro-annuli that no other product can reach, sealing leaks at their source.
3. Once in place, the fluid begins its transformation into crystalline calcium carbonate – a limestone-like material that binds to rock, cement, and steel, permanently sealing any and all leakage pathways.
4. Crystal formation persists for several hours after the organisms become inactive, quickly creating an impermeable and permanent seal. Once solid, the calcium carbonate is stronger than the existing formation.
The Result: The Most Effective Solution For Stopping Methane Leaks
Using biomineralization, BioSqueeze® creates a cost-effective, permanent seal, eliminating sustained casing pressure consistently. For those pioneering stewardship of the Earth, BioSqueeze® is carbon neutral, from production to sealing. Due to its natural composition, it can be used safely where other solutions can’t, including sensitive areas near aquifers and water tables.
Our Process:
The Standard BioSqueeze® Process
In most wells, our bottom annular gas squeeze is used to permanently eliminate sustained annular pressure. Here’s how we do it.
1. Operator identifies leakage pathways through bond logging.
2. Operator places bridge plug and perforates or notches casing to target specific zones where leakage pathways can be accessed.
3. The first day, the BioSqueeze crew connects its equipment and pumps low-viscosity, environmentally friendly biomineralization solution in position downhole using tubing.
4. BioSqueeze pumps fluids into problem annuli for about 6 hours, applying pressure to squeeze fluids deep into leakage pathways. Meanwhile, our crew monitors the injection rate and pressure until gas migration is eliminated.
5. BioSqueeze seals the well in overnight at pressure to continue to form the barrier.
6. The following day, the BioSqueeze crew returns to continue pumping fluids, ensuring that all problem annuli are fully sealed.
One Effective Solution, Many Applications
BioSqueeze® can be applied to oil and gas wells in various stages of production. Three different processes target specific problems to make sure sustained annular pressure is eliminated for good in very few applications. Learn more about our solutions.
APPLICATIONS:
One Effective Solution, Many Applications
BioSqueeze® can be applied to oil and gas wells in various stages of production. Three different processes target specific problems to make sure sustained annular pressure is eliminated for good in the very few applications. Learn more about our solutions.
BioSqueeze® Applications:
a) Plug & Abandon (P&A) Wells
Eliminate Sustained Annular Pressure in P&A Wells
When it’s time to retire an oil and gas well for good, BioSqueeze® permanently eliminates methane leakage and sustained annular pressure in the well without having to return several times to meet regulations. For P&A jobs where a cement plug in the wellbore has already failed, BioSqueeze® can seal cracks in the cement plug to eliminate leaks without the need for any drill out.
BioSqueeze® can eliminate sustained annular pressure in plug and abandon wells using three different techniques, shown below.
- Downhole BioSqueeze®
Operator Preparation
Step 1:
Identify problem annulus through bond logging.
Step 2:
Place bridge plug and perforate or notch casing to target leakage pathways.
Step 3:
Set packer and run tubing to bottom perf/notch to squeeze into the annulus.
BioSqueeze® Execution
Step 4:
The first day, connect equipment and pump low-viscosity, environmentally friendly biomineralization solution in position downhole using tubing.
Step 5:
Pump fluids into problem annuli for about 6 hours, applying pressure to squeeze fluids deep into leakage pathways. Monitor injection rate and pressure until gas migration is eliminated.
Step 6:
Seal the well in overnight at pressure to continue to form the barrier.
Step 7:
The following day, return to continue pumping fluids, ensuring that all problem annuli are fully sealed.
- Deep Penetrating Annular Surface (DPAS) BioSqueeze®
Operator Preparation
Step 1:
Identify problem annuli and identify top of cement.
BioSqueeze® Execution
Step 2:
The first day, connect equipment and pump low-viscosity, environmentally friendly biomineralization solution into the cement at the top of the problem annulus.
Step 3:
Pump fluids into the annulus for about 6 hours, applying pressure to squeeze fluids deep into leakage pathways. Monitor injection rate and pressure until gas migration is eliminated.
Step 4:
Seal the well in overnight at pressure to continue to form the barrier.
Step 5:
The following day, return to continue pumping fluids, ensuring that micro annuli are fully sealed.
- Cement Top BioSqueeze®
Operator Preparation
Step 1:
Set packer and run tubing to just above existing cement plug.
BioSqueeze® Execution
Step 2:
The first day, connect equipment and pump low-viscosity, environmentally friendly biomineralization solution into the leaking cement plug.
Step 3:
Pump fluids into the wellbore for about 6 hours, applying pressure to squeeze fluids deep into leakage pathways. Monitor injection rate and pressure until gas migration is eliminated.
Step 4:
Seal the well in overnight at pressure to continue to form the barrier.
Step 5:
The following day, return to continue pumping fluids, ensuring that all micro annuli are fully sealed.
b) Drilled But Uncompleted (DUC) Wells
Eliminate Sustained Casing Pressure in DUC Wells
Oil and gas wells that have been drilled but have not yet undergone various completion activities are often compromised by annular leaks. Pressure from these leaks continues to build over time, with the risk of a blowout unless vented or flared. BioSqueeze® can help operators avoid band-aid solutions, eliminating sustained casing pressure in very few attempts, foregoing the need for maintenance down the road. Since BioSqueeze® seals leakage pathways themselves and leaves nothing in the wellbore, no-drill out is needed after the job is done, saving operators time and money.
BioSqueeze® can eliminate sustained casing pressure in DUC wells using a Bottom Annular Gas Squeeze, shown below.
Downhole BioSqueeze®
Operator Preparation
Step 1:
Identify problem annulus through bond logging.
Step 2:
Place bridge plug and perforate or notch casing to target leakage pathways.
Step 3:
Set packer and run tubing to bottom perf/notch to squeeze into the annulus.
BioSqueeze® Execution
Step 4:
The first day, connect equipment and pump low-viscosity, environmentally friendly biomineralization solution in position downhole using tubing.
Step 5:
Pump fluids into problem annuli for about 6 hours, applying pressure to squeeze fluids deep into leakage pathways. Monitor injection rate and pressure until gas migration is eliminated.
Step 6:
Seal the well in overnight at pressure to continue to form the barrier.
Step 7:
The following day, return to continue pumping fluids, ensuring that all problem annuli are fully sealed.
c) Producing Wells
Eliminate Bradenhead Pressure in Producing Wells
Wells that currently produce oil and gas can create unwanted bradenhead pressure (sustained casing pressure), resulting in methane emissions. BioSqueeze® can seal leakage pathways so that the well can keep producing at a high rate, without unnecessary emissions escaping in the process. Since BioSqueeze® seals leakage pathways themselves and leaves nothing in the wellbore, no drill out is needed after the job is done, saving operators time and money.
BioSqueeze® can eliminate bradenhead pressure in producing wells using a Bottom Annular Gas Squeeze, shown below.
- Downhole BioSqueeze®
Operator Preparation
Step 1:
Identify problem annulus through bond logging.
Step 2:
Place bridge plug and perforate or notch casing to target leakage pathways.
Step 3:
Set packer and run tubing to bottom perf/notch to squeeze into the annulus.
BioSqueeze® Execution
Step 4:
The first day, connect equipment and pump low-viscosity, environmentally friendly biomineralization solution in position downhole using tubing.
Step 5:
Pump fluids into problem annuli for about 6 hours, applying pressure to squeeze fluids deep into leakage pathways. Monitor injection rate and pressure until gas migration is eliminated.
Step 6:
Seal the well in overnight at pressure to continue to form the barrier.
Step 7:
The following day, return to continue pumping fluids, ensuring that all problem annuli are fully sealed.
d) Gas Storage Wells
Eliminate Methane Leaks in Gas Storage Wells
Keep your valuable resources from escaping by sealing problem leaks at their source. BioSqueeze®'s biomineralization solution can seal any leakage pathways effectively, allowing you to retain more of your profit from gas storage wells. Without no need to drill out before or after, you'll save time money by using the most effective solution first.
BioSqueeze® uses a Bottom Annular Gas Squeeze to seal methane leaks in gas storage wells, shown below.
- Downhole BioSqueeze®
Operator Preparation
Step 1:
Identify problem annulus through bond logging.
Step 2:
Place bridge plug and perforate or notch casing to target leakage pathways.
Step 3:
Set packer and run tubing to bottom perf/notch to squeeze into the annulus.
BioSqueeze® Execution
Step 4:
The first day, connect equipment and pump low-viscosity, environmentally friendly biomineralization solution in position downhole using tubing.
Step 5:
Pump fluids into problem annuli for about 6 hours, applying pressure to squeeze fluids deep into leakage pathways. Monitor injection rate and pressure until gas migration is eliminated.
Step 6:
Seal the well in overnight at pressure to continue to form the barrier.
Step 7:
The following day, return to continue pumping fluids, ensuring that all problem annuli are fully sealed.
e) Carbon Sequestration
Eliminate Annular Leaks in Carbon Sequestration Wells
Carbon capture, utilization and sequestration (CCUS) is becoming an important solution for reducing the impact of the oil and gas industry on the planet. BioSqueeze® can make Class VI injection wells more efficient at storing carbon dioxide by eliminating sustained casing pressure and sealing leakage pathways at their source. Since BioSqueeze® seals leakage pathways themselves and leaves nothing in the wellbore, no drill out is needed after the job is done, saving operators time and money.
BioSqueeze® uses its Bottom Annular Gas Squeeze to seal leaks in carbon sequestration wells, shown below.
Downhole BioSqueeze®
Operator Preparation
Step 1:
Identify problem annulus through bond logging.
Step 2:
Place bridge plug and perforate or notch casing to target leakage pathways.
Step 3:
Set packer and run tubing to bottom perf/notch to squeeze into the annulus.
BioSqueeze® Execution
Step 4:
The first day, connect equipment and pump low-viscosity, environmentally friendly biomineralization solution in position downhole using tubing.
Step 5:
Pump fluids into problem annuli for about 6 hours, applying pressure to squeeze fluids deep into leakage pathways. Monitor injection rate and pressure until gas migration is eliminated.
Step 6:
Seal the well in overnight at pressure to continue to form the barrier.
Step 7:
The following day, return to continue pumping fluids, ensuring that all problem annuli are fully sealed.
What we do
Methane leaks in oil and gas wells cost the industry millions of dollars in unsuccessful attempts to seal while taking up precious time and resources. Historically, technologies for eliminating sustained casing pressure have been inconsistent, expensive, and inefficient. BioSqueeze® sets a new standard as the most effective and environmentally friendly solution for stopping gas migration.
BioSqueeze® answers the call with a cost-effective solution that seals even the toughest leaks at a single flat rate.
Eliminate Sustained Casing Pressure, Once and For All
BioSqueeze Inc. has successfully sealed leaks in nine U.S. states and two Canadian provinces – often eliminating leaks in multiple annuli in a single application. BioSqueeze® has been used in P&A, DUC and producing wells, as well as gas storage and sequestration wells. Avoid paying for another round of drill out and cement, costing hundreds of thousands of dollars and tying up crews for months on end. If we don’t stop the leak on our first attempt, we will work with your team until the problem is solved.
Why Choose Biosqueeze®?
- Sealed Well
BioSqueeze® routinely seals multiple leaks in a single application – often after dozens of other attempts using cement and resins have failed and millions have already been spent. BioSqueeze® does the job right, the first time.
- Superior Penetration
Low viscosity (1.05 cP) fluids seal even the smallest leakage pathways (as small as 1 micron or 0.00008 inches wide), which no other technology can reach.
- Permanent, Gas-Tight Seal
BioSqueeze® forms crystalline calcium carbonate similar to limestone in leakage pathways, readily bonding to steel, cement, and the formation to create an impermeable seal that prevents leaks once and for all.
- No Drill Out Necessary
BioSqueeze® forms a seal in the leakage pathways themselves, not the wellbore, leaving nothing to drill out after the job is done.
- Green Technology
BioSqueeze® uses natural and safe biomineralization technology, which poses no risk for aquifer contamination and generates less than 1/3 the amount of CO2 as a traditional cement squeeze.
- Abundant Applications
BioSqueeze® seals leaks to eliminate annular casing pressure in a wide variety of wells, before or after other attempts to seal and across a wide range of temperatures, pressures and depths. BioSqueeze® can also be used in refracturing and enhanced oil recovery to utilize more of a well's potential.
A). MacroSeal™
In applications where cement bond is poor but present remedial cementing can be ineffective as placement may be impossible, cement can be gas cut before it sets, and the frac gradient is often exceeded thus exacerbating existing integrity issues by creating additional leakage pathways.
MacroSeal™ is a low-viscosity fluid system that rapidly crystalizes to form an impermeable, limestone barrier stronger than existing cement. MacroSeal™ can be injected into poorly cemented intervals, is impervious to gas cut, and is placed with precision pumping systems that ensure the frac gradient is not exceeded making it a more effective solution for restoring cement integrity.
4x More Successful than Cement

B). MicroSeal™
In applications where leakage pathways are too tight for remedial cementing alternative sealants like resins and micro-fine cements can be ineffective due to their inability to access all leakage pathways and shrinkage that occurs during setup allowing gas to circumvent the new barrier, with the status quo being repeated remediations and subsequent drillout.
MicroSeal™ is a low-viscosity fluid system that self-diverts into leakage pathways to repair existing barriers. MicroSeal™ can be injected into channels, micro-annuli, and pinholes where it forms a gas-tight seal while leaving the wellbore full drift, thus reducing risk and making it the most effective and economic solution on the market.
3x More Successful than Resins

C). NanoSeal™
In applications where cement bond is good and injection cannot be established but small leaks exist/persist options are limited to extensive remediations to establish a new barrier entirely or intrusive treatments that mechanically alter existing cement. Both fail to address the root cause while incurring significant costs and risks.
NanoSeal™ is a low-viscosity fluid system composed of sub-micron particles that penetrates deep into the smallest aperture gaps to eliminate fluid/gas migration. NanoSeal™ is safe for application in environmentally sensitive applications, capable of penetrating 1,000s of ft, and strengthens the materials its injected into making it the only solution for healing defects in otherwise competent cement.
The Only Solution for Sealing Sub-Micron Leaks

Life at BioSqueeze
Our Values:
- Integrity
We prioritize integrity in everything we do. We put our clients first, maintaining honesty and transparency in all of our interactions.
- Innovation
Continued innovation drives us to be the world leader in biomineralization applications.
- Environmentally Responsible
We work to create a healthier planet by harnessing the power of biomineralization – a natural process for solving problems faced by the oil and gas industry.
- Work Ethic
Our strong work ethic emanates from our Montana roots. We continue until our work is done, no matter what it takes.
- Success
Our success is defined by solving our clients’ problems. When our clients are satisfied, we are satisfied.
- Safety-Minded
We keep our safety standards high to make sure everyone out in the field goes home safe at night.

